Effect of waste mixed with biochar as soil amendment on trace element solubility in a mine soil


Abandoned mining sites often cause contamination of surface and subsurface waters. A 3-month pot experiment was performed to evaluate the influence of waste mixed with biochar as a soil amendment on a series of chemical characteristics and trace element solubility in a mine soil. Trace element concentrations were measured in pore water of the mine soil reclaimed with different proportions of waste-biochar amendment (20, 40, 80 and 100%) and grown with Brassica juncea. The results showed that amendment application improved soil conditions such as pH, total carbon, dissolved organic carbon, total nitrogen, and strongly reduced the concentration of Al (99.99%), Co (99.95%), Cu (99.97%), Fe (99.79%) and Ni (99.91%) in pore water, compared to the unamended soils. Waste and biochar also promoted the establishment of B. juncea in the mine soil. These results highlight the importance of mitigating the impacts from abandoned mines sites on water quality. The use of waste and biochar as soil amendment combined with B. juncea plants was effective in reducing metal concentrations in pore water and the associated toxicity risk.



Agegnehu G, Bass AM, Nelson PN, Muirhead B, Wright G, Bird MI. 2015. Biochar and biochar-compost as soil amendments: Effects on peanut yield, soil properties and greenhouse gas emissions in tropical North Queensland, Australia. Agric Ecosyst Environ 213:72-85.

Azubuike CC, Chikere CB, Okpokwasili GC. 2016. Bioremediation techniques–classification based on site of application: principles, advantages, limitations and prospects. World J Microbiol Biotechnol. 32:180.

Beane SJ, Comber SDW, Rieuwerts J, Long P. 2016. Abandoned metal mines and their impact on receiving waters: A case study from Southwest England. Chemosphere 153:294-306.

Beesley L, Dickinson N. 2011. Carbon and trace element fluxes in the pore water of an urban soil following greenwaste compost, woody and biochar amendments, inoculated with the earthworm Lumbricus terrestris. Soil Biol Biochem. 43:188-196.

Beesley L, Inneh OS, Norton GJ, Moreno-Jiménez E, Pardo T, Clemente R, Dawson JJC. 2014. Assessing the influence of compost and biochar amendments on the mobility and toxicity of metals and arsenic in a naturally contaminated mine soil. Environ Pollut. 186:195-202.

Beesley L, Moreno-Jiménez E, Clemente R, Lepp N, Dickinson N. 2010a. Mobility of arsenic, cadmium and zinc in a multi-element contaminated soil profile assessed by in-situ soil pore water sampling, column leaching and sequential extraction. Environ Pollut. 158(1):155-160.

Beesley L, Moreno-Jiménez E, Gomez-Eyles JL. 2010b. Effects of biochar and greenwaste compost amendments on mobility, bioavailability and toxicity of inorganic and organic contaminants in a multi-element polluted soil. Environ Pollut. 158:22827-2287.

Beesley L, Moreno-Jiménez E, Gomez-Eyles JL, Harris E, Robinson B, Sizmur T. 2011. A review of biochars’ potential role in the remediation, revegetation and restoration of contaminated soils. Environ Pollut. 159:3269-3282.

Bolan NS, Kunhikrishnan A, Naidu R. 2013. Carbon storage in a heavy clay soil landfill site after biosolid application. Sci Total Environ. 465:216-225.

Bolan N, Kunhikrishnan A, Thangarajan R, Kumpiene J, Park J, Makino T, Kirkham MB, Scheckel K. 2014. Remediation of heavy metal(- loid)s contaminated soils—to mobilize or to immobilize? J Hazard Mater. 266:141-166.

Food and Agriculture Organization of the United Nations (FAO). 2014. World Reference Base for Soil Resources. Rome: FAO.

Fresno T, Moreno-Jiménez E, Peñalosa JM. 2016. Assessing the combination of iron sulfate and organic materials as amendment for an arsenic and copper contaminated soil. A chemical and ecotoxicological approach. Chemosphere 165:539-546.

Hansen V, Müller-Stöver D, Munkholm LJ, Peltre C, Hauggaard-Nielsen H, Jensen LS. 2016. The effect of straw and wood gasification biochar on carbon sequestration, selected soil fertility indicators and functional groups in soil: An incubation study. Geoderma 269:99-107.

Hattab N, Motelica-Heino M, Faure O, Bouchardon J-L. 2015. Effect of fresh and mature organic amendments on the phytoremediation of technosols contaminated with high concentrations of trace elements. J Environ Manage. 159:37-47.

Kabata-Pendias A. 2004. Soil-plant transfer of trace elements–an environmental issue. Geoderma 122:143-149.

Kabata-Pendias A. 2011. Trace Elements in Soils and Plants. 4th edition. Boca Raton, FL, USA: CRC Press.

Karami N, Clemente R, Moreno-Jiménez E, Lepp NW, Beesley L. 2011. Efficiency of green waste compost and biochar soil amendments for reducing lead and copper mobility and uptake to ryegrass. J Hazard Mater. 191:41-48.

Kumpiene J, Lagerkvist A, Maurice C. 2008. Stabilization of As, Cr, Cu, Pb and Zn in soil using amendments–A review. Waste Manage. 28:215-225.

Laird DA, Fleming P, Davis DD, Horton R, Wang B, Karlen DL. 2010. Impact of biochar amendments on the quality of a typical Midwestern agricultural soil. Geoderma 158:443-449.

Lamb DT, Heading S, Bolan N, Naidu R. 2012. Use of biosolids for phytocapping of landfill soil. Water Air Soil Pollut. 223:2695-2705.

Lehmann J. 2007. Bio-energy in the black. Front Ecol Environ. 5:381-387.

Liu Z, Zhang FS. 2009. Removal of lead from water using biochars prepared from hydrothermal liquefaction of biomass. J Hazard Mat. 167:933-939.

Liu W, Wang S, Lin P, Sun H, Hou J, Zuo Q, Huo R. 2016a. Response of CaCl2-extractable heavy metals, polychlorinated biphenyls, and microbial communities to biochar amendment in naturally contaminated soils. J Soils Sediments 16:476-485.

Liu C, Wang H, Tang X, Guan Z, Reid BJ, Rajapaksha AU, Ok YS, Sun H. 2016b. Biochar increased water holding capacity but accelerated organic carbon leaching from a sloping farmland soil in China. Environ Sci Pollut Res. 23:995-1006.

Ma Y, Rajkumar M, Rocha I, Oliveira RS, Freitas H. 2015. Serpentine bacteria influence metal translocation and bioconcentration of Brassica juncea and Ricinus communis grown in multi-metal polluted soils. Front Plant Sci. 5:757.

Mahar A, Wang P, Ali A, Awasthi MK, Lahori AH, Wang Q, Li R, Zhang Z. 2016. Challenges and opportunities in the phytoremediation of heavy metals contaminated soils: A review. Ecototoxicol Environ Saf. 126:111-121.

Manzano R, Peñalosa JM, Esteban E. 2014. Amendment application in a multicontaminated mine soil: Effects on trace element mobility. Water Air Soil Poll. 225:1874.

Mench M, Lepp N, Bert V, Schwitzguebel J-P, Gawronski SW, Schöder P, Vangronsveld J. 2010. Successes and limitations of phytotechnologies at field scale: outcomes, assessment and outlook from COST Action 859. J Soils Sediments 10:1039-1070.

Mingorance MD, Rossini Oliva S, Valdés B, Pina Gata FJ, Leidi EO, Guzmán I, Peña A. 2014. Stabilized municipal sewage sludge addition to improve properties of an acid mine soil for plant growth. J Soils Sediments 14:703-715.

Mosley LM, Willson P, Hamilton B, Butler G, Seaman R. 2015. The capacity of biochar made from common reeds to neutralize pH and remove dissolved metals in acid drainage. Environ Sci Pollut Res. 22:15113-15122.

Mourato MP, Moreira IN, Leitão I, Pinto FR, Sales JR, Martins LL. 2015. Effect of heavy metals in plants of the genus Brassica. Int J Mol Sci. 16(8):17975-17998.

Neilson S, Rajakaruna N. 2012. Roles of rhizospheric processes and plant physiology in applied phytoremediation of contaminated soils using Brassica oilseeds. In: Anjum NA, Ahmad I, Pereira ME, Duarte AC, Umar S, Khan NA, editors. The plant family Brassicaceae, 21. Dordrecht: Springer Netherlands. p. 313-330.

Novak JM, Busscher WJ, Laird DL, Ahmedna M, Watts DW, Niandou MAS. 2009. Impact of biochar amendment on fertility of a southeastern coastal plain soil. Soil Sci. 174:105-112.

Pardo T, Clemente R, Epelde L, Garbisu C, Bernal MP. 2014. Evaluation of the phytostabilisation efficiency in a trace elements contaminated soil using soil health indicators. J Hazard Mater. 268:68-76.

Park JH, Choppala GK, Bolan NS, Chung JW, Chuasavathi T. 2011. Biochar reduces the bioavailability and phytotoxicity of heavy metals. Plant Soil 348:439-451.

Peltre C, Nyord T, Bruun S, Jensen LS, Magid J. 2015. Repeated soil application of organic waste amendments reduces draught force and fuel consumption for soil tillage. Agric Ecosyst Environ. 211:94-101.

Peña A, Mingorance MD, Guzmán-Carrizosa I, Fernández-Espinosa AJ. 2015. Improving the mining soil quality for a vegetation cover after addition of sewage sludges: Inorganic ions and low-molecular-weight organic acids in the soil solution. J Environ Manage. 150:216-225.

Pérez-Esteban J, Escolástico C, Moliner A, Masaguer A, Ruiz-Fernández J. 2014. Phytostabilization of metals in mine soils using Brassica juncea in combination with organic amendments. Plant Soil 377:97-109.

Porta J. 1986. Técnicas y experimentos en Edafología. Barcelona, Spain: Collegi Oficial D`Enginyers Agronoms de Catalunya.

Puga AP, Abreu CA, Melo LCA, Paz-Ferreiro J, Beesley L. 2015. Cadmium, lead, and zinc mobility and plant uptake in a mine soil amended with sugarcane straw biochar. Environ Sci Pollut Res. 22:17606-17614.

Puga AP, Melo LCA, de Abreu CA, Coscione AR, Paz-Ferreiro J. 2016. Leaching and fractionation of heavy metals in mining soils amended with biochar. Soil Till Res. 164:25-33.

Puig CG, Álvarez-Iglesias L, Reigosa MJ, Pedrol N. 2013. Eucalyptus globulus leaves incorporated as green manure for weed control in maize. Weed Sci. 61:154-161.

Rascio N, Navari-Izzo F. 2011. Heavy metal hyperaccumulating plants: how and why do they do it? And what makes them so interesting? Plant Sci. 180:169-181.

Roberts DA, Cole AJ, Paul NA, de Nys R. 2015. Algal biochar enhances the re-vegetation of stockpiled mine soils with native grass. J Environ Manage. 161:173-180.

Ruttens A, Mench M, Colpaert JV, Boisson J, Carleer R, Vangronsveld J. 2006. Phytostabilization of a metal contaminated sandy soil. I: influence of compost and/or inorganic metal immobilizing soil amendments on phytotoxicity and plant availability of metals. Environ Pollut. 144:524e532.

Sakaguchi I, Inoue Y, Nakamura S, Kojima Y, Sasai R, Sawada K, Suzuki K, Takenaka C, Katayama A. 2015. Assessment of soil remediation technologies by comparing health risk reduction and potential impacts using unified index, disability-adjusted life years. Clean Techn Environ Policy 17:1663-1670.

Sánchez-Monedero MA, Roig A, Martínez-Pardo C, Cegarra J, Paredes C. 1996. A microanalysis method for determining total organic carbon in extracts of humic substances. Relationships between total organic carbon and oxidable carbon. Bioresour Technol. 57:291-295.

Sherene T. 2009. Effect of dissolved organic carbon (DOC) on heavy metal mobility in soils. Nat Environ Pollut Technol. 8:817-821.

Sombroek W, Ruivo ML, Fearnside PM, Glaser B, Lehmann J. 2003. Amazonian Dark Earths as carbon stores and sinks. In: Lehmann J, Kern DC, Glaser B, Woods WI, editors. Amazonian Dark Earths: origin, properties, management. Dordrecht, Netherlands: Kluwer Academic Publishers. p. 125-139.

Thakur S, Sharma SS. 2016. Characterization of seed germination, seedling growth, and associated metabolic responses of Brassica juncea L. cultivars to elevated nickel concentrations. Protoplasma 253:571-580.

Thakur S, Singh L, Wahid ZA, Siddiqui MF, Atnaw SM, Din MFM. 2016. Plant-driven removal of heavy metals from soil: uptake, translocation, tolerance mechanism, challenges, and future perspectives. Environ Monit Assess. 188:206.

Uchimiya M, Lima IM, Klasson KT, Wartelle LH. 2010. Contaminant immobilization and nutrient release by biochar soil amendment: Roles of natural organic matter. Chemosphere 80:935-940.

USDA. 1998. Soil quality indicators: pH. https://www.nrcs.usda.gov/Internet/FSE_DOCUMENTS/nrcs142p2_052208.pdf.

Wood JL, Tang C, Franks AE. 2016. Microbial associated plant growth and heavy metal accumulation to improve phytoextraction of contaminated soils. Soil Biol Biochem. 103:131-137.

Yin B, Zhou L, Yin B, Chen L. 2016. Effects of organic amendments on rice (Oryza sativa L.) growth and uptake of heavy metals in contaminated soil. J Soils Sediments 16:537-546.

Zeng G, Wu H, Liang J, Guo S, Huang L, Xu P, Liu Y, Yuan Y, He X, He Y. 2015. Efficiency of biochar and compost (or composting) combined amendments for reducing Cd, Cu, Zn and Pb bioavailability, mobility and ecological risk in wetland soil. RSC Advances 5:34541-34548.

Zhang Y, Tang X, Luo W. 2014. Metal removal with two biochars made from municipal organic waste: adsorptive characterization and surface complexation modeling. Toxicological and Environmental Chemistry 96:1463-1475.

Zong Y, Xiao Q, Lu S. 2016. Acidity, water retention, and mechanical physical quality of a strongly acidic Ultisol amended with biochars derived from different feedstocks. J Soils Sediments 16:177-190.

Zornoza R, Acosta JA, Faz A, Bååth E. 2016. Microbial growth and community structure in acid mine soils after addition of different amendments for soil reclamation. Geoderma 272:64-72.